Recently, the Hortus Botanicus has expressed its interest in the use of native plant species in the renovation, in order to increase its biodiversity. Both the environmental requirements and the interests of different stakeholders should be accounted for with the selection of native plant species. The aim of this study is to provide a sound scientific basis to determine which native plant species can best be used in UGS, applying as case-study the Hortusplantsoen, in Amsterdam. To achieve our aim, we applied a stepwise approach to (i) map out the current habitat conditions, (ii) structure the expectations of the stakeholders for the use of native plant species, (iii) determine the ecological requirements of prospective native plant species and to (iv) match ecological requirements with and the stakeholder The finding of this study will lead to a recommendation of native plant species to use in the Hortusplantsoen, Amsterdam. My first advice is to use humus with the renovation of the Hortusplantsoen. My next advice is to use the listed ten best suited species for the habitat type. Even though there are some disadvantages in this method, it would still be best for the selection of native plant species. This research has contributed directly to the project of Knowledge Mile Park by giving advice on the use of native plant species in the refurbishment of the Hortusplantsoen. This research presents an objective and structured scientific method to use when selecting plant species for the use of UGS in similar project. This could stimulate the use of native plant species in UGS, which could also increase the biodiversity of fauna in these areas.